✅ Neutral Protease – Mild Protein Hydrolyzing Enzyme for Food, Pharma, and Biotech

Fermentation-derived protease (≥200,000 U/g) active in neutral pH, ideal for hydrolysis of proteins in food, beverage, and fermentation systems.

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🌿 Product Overview

Neutral Protease is a non-specific endopeptidase that hydrolyzes peptide bonds in a broad range of proteins. Produced via microbial fermentation (e.g. Bacillus subtilis), Chibio’s Neutral Protease 200,000 U/g performs best under neutral pH (6.0–7.5) and moderate temperature conditions (40–55°C). It is widely used in gelatin hydrolysis, flavor production, brewing, soy fermentation, protein pre-treatment, and peptide preparation.

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🔍 Basic Information

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🧪 Specification (Based on COA)​

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💧 Physicochemical Properties

• ✔️ Broad substrate specificity

• ✔️ Mild enzymatic hydrolysis at neutral pH

• ✔️ Non-GMO, vegan, allergen-free

• ✔️ Compatible with other enzymes like papain, pepsin, trypsin

• ✔️ Low bitterness in protein hydrolysates

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🎯 Key Benefits

✅ Ideal for preparing bioactive peptides from soy, dairy, or animal protein
✅ Efficient protein breakdown for food flavor or fermentation enhancement
✅ Mild processing conditions – protects sensitive bioactives
✅ Free of harsh chemical residues – clean-label compatible
✅ Enhances digestibility and reduces viscosity

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🌐 Applications & Dosage Forms

🍲 Protein Hydrolysates (Plant or Animal-Based)

• Use: Produces low-bitter peptides for nutrition and flavor

• Dosage: 0.1–0.5% based on protein content

• Result: Improved solubility, functionality, taste profile

🍜 Seasonings & Fermented Protein Sauces

• Use: Hydrolyzes soy, wheat, fish proteins into savory base

• Dosage: 100–500 U/kg substrate

• Effect: Increases glutamate/nucleotide content naturally

🍺 Brewing & Alcohol Fermentation

• Use: Protein stabilization, wort clarification

• Dosage: 50–200 U/kg malt or adjunct

• Benefit: Improves clarity, foam, and fermentation yield

💊 Peptide Nutritional Supplements

• Use: Produces bioavailable peptides for digestive, immune health

• Form: Spray-dried or encapsulated formulations

• Dosage: Varies with protein and target peptide profile

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🔄 Neutral Protease Production Flowchart

Microbial Fermentation
→ Cultivation of selected Bacillus strain in sterile bioreactors

Enzyme Recovery
→ Filtration and purification of enzyme-rich broth

Concentration & Drying
→ Vacuum evaporation or ultrafiltration → Spray-dried into powder

Stabilization
→ Optional carrier blend (maltodextrin, rice starch)

Quality Control
→ Activity (U/g), heavy metals, microbiology, moisture testing

Packaging
→ Food/pharma-grade drum packaging under sealed, dry conditions

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💼 Why Choose Chibio Biotech?

🧬 Broad-Spectrum Protease Portfolio

We offer acid, neutral, and alkaline proteases for full hydrolysis control

📊 High Enzyme Potency

≥200,000 U/g activity ensures lower dosage and stronger efficiency

🌿 Clean-Label Compliant

Non-GMO, vegan-friendly, suitable for flavor and nutrition systems

📦 Scalable Supply Chain

From lab batches to industrial bulk with global logistics and OEM support

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💡 Why Use Neutral Protease in Business?

Chibio’s Neutral Protease helps businesses maximize protein value while keeping formulations natural, efficient, and marketable:

• Mild Enzymatic Action: Protects sensitive peptides, avoids over-hydrolysis

• Versatile Applications: Used across flavor, fermentation, and nutrition sectors

• Stable Performance: Works in pH-neutral, heat-sensitive processing lines

• Economic Use: High enzyme strength reduces formulation cost per unit

✅ Whether you’re crafting seasonings, functional peptides, or fermentation bases – Chibio’s Neutral Protease brings precision and purity.

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📩 Request Free Sample or Quotation Now!

Looking for high-purity Neutral Protease for food, supplement, or industrial applications?

📧 Email: sales@chibiotech.com
📞 Phone: +86 (0)532 66983270
🌐 Website: www.chibiotech.com

✅ Chibio Biotech – Natural Enzymes for Advanced Protein Solutions